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The crystal structure of a lectin from Butea monosperma: insight into its glycosylation and binding of ligands.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2015 Jan; Vol. 72, pp. 1376-83. - Publication Year :
- 2015
-
Abstract
- Crystal structure of a lectin purified from Butea monosperma seeds was determined by Molecular Replacement method. Its primary structure was determined by Tandem Mass Spectroscopy and electron density maps from X-ray diffraction data. Its quaternary structure was tetrameric, formed of two monomers, α and β, β appearing as truncated α. The occurrence of two tetramers in the asymmetric unit of the crystal might be a consequence of asymmetric contacts due to difference in glycosylation and variable loops structures, to form an 'octamer-structure'. The crystal structure showed binding pockets for γAbu, having a proposed role in plant defense, at the interface of canonical dimer-partners. Hemagglutination studies, enzyme kinetics, isothermal titration calorimetry and molecular dynamics showed that the lectin is specific to N-acetyl d-galactosamine, galactose and lactose in decreasing order, and α-amylase inhibitor.
- Subjects :
- Amino Acid Sequence
Binding Sites
Calorimetry
Crystallography, X-Ray
Glycerol metabolism
Glycosylation
Ions
Kinetics
Ligands
Mass Spectrometry
Metals chemistry
Molecular Dynamics Simulation
Molecular Sequence Data
Molecular Weight
Oligosaccharides chemistry
Plant Lectins pharmacology
Protein Conformation
Solvents
alpha-Amylases antagonists & inhibitors
alpha-Amylases metabolism
gamma-Aminobutyric Acid chemistry
gamma-Aminobutyric Acid metabolism
Butea chemistry
Plant Lectins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 72
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 25316424
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2014.10.004