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Diazotrophic potential among bacterial communities associated with wild and cultivated Agave species.
- Source :
-
FEMS microbiology ecology [FEMS Microbiol Ecol] 2014 Dec; Vol. 90 (3), pp. 844-57. Date of Electronic Publication: 2014 Nov 04. - Publication Year :
- 2014
-
Abstract
- Agaves are major biotic resources in arid and semi-arid ecosystems. Despite their ecological, economical and cultural relevance, many aspects of the microbial communities associated with agaves are still unknown. Here, we investigated the bacterial communities associated with two Agave species by 16S rRNA- Denaturing gradient gel electrophoresis fingerprinting and sequencing. We also evaluated the effects of biotic and abiotic factors in the structure of the bacterial communities. In parallel, we isolated and characterized diazotrophic bacteria associated with agaves, as Agave soils are characterized by their low nitrogen content. Our results demonstrate that in Agave, the structure of prokaryotic assemblages was mostly influenced by the community group, where the soil, episphere, and endosphere were clearly distinct. Proteobacteria (γ and α), Actinobacteria, and Acidobacteria were the dominant phyla. Bacterial communities in the episphere of agaves were mainly influenced by the host species, whereas in the endosphere were affected by the season. Fifteen bacterial taxa were common and abundant in the endosphere of both Agave species during the dry season. Notably, some of the confirmed diazotrophic strains belonged to this group, suggesting a possible beneficial role in planta.<br /> (© 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.)
- Subjects :
- Acidobacteria genetics
Acidobacteria isolation & purification
Actinobacteria genetics
Actinobacteria isolation & purification
Base Sequence
Denaturing Gradient Gel Electrophoresis
Ecosystem
Microbial Consortia genetics
Nitrogen analysis
Nitrogen Fixation
Proteobacteria genetics
Proteobacteria isolation & purification
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Soil chemistry
Soil Microbiology
Acidobacteria classification
Actinobacteria classification
Agave microbiology
Proteobacteria classification
Subjects
Details
- Language :
- English
- ISSN :
- 1574-6941
- Volume :
- 90
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEMS microbiology ecology
- Publication Type :
- Academic Journal
- Accession number :
- 25314594
- Full Text :
- https://doi.org/10.1111/1574-6941.12438