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Sorghum (Sorghum bicolor).
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1223, pp. 181-8. - Publication Year :
- 2015
-
Abstract
- Agrobacterium-mediated transformation of sorghum (Sorghum bicolor L. Moench) targeting immature embryo explants is a route to introduce transgenic alleles into the crop. The protocol requires maintenance of quality stock plants under greenhouse conditions for a constant supply of immature embryo explants. This is typically carried out by a regular sowing of seeds, minimal use of pesticides, and monitoring of plants to document pollen dispersal and bagging of heads. The time frame from explant inoculation to establishment of a primary transgenic event in the greenhouse typically ranges from 4 to 6 months. Seed set in the primary transformants is comparable to greenhouse-grown stock plants, with the majority of the transgenic alleles being inherited as a single functional locus.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1223
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 25300840
- Full Text :
- https://doi.org/10.1007/978-1-4939-1695-5_14