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Expression of classical components of the renin-angiotensin system in the human eye.
- Source :
-
Journal of the renin-angiotensin-aldosterone system : JRAAS [J Renin Angiotensin Aldosterone Syst] 2015 Mar; Vol. 16 (1), pp. 59-66. Date of Electronic Publication: 2014 Oct 06. - Publication Year :
- 2015
-
Abstract
- Purpose: The purpose of this study was to determine the relative expression of clinically-relevant components of the renin-angiotensin system (RAS) in the adult human eye.<br />Methods: We obtained 14 post-mortem enucleated human eyes from patients whom had no history of inflammatory ocular disease nor pre-mortem ocular infection. We determined the gene expression for prorenin, renin, prorenin receptor, angiotensin-converting enzyme, angiotensinogen and angiotensin II Type 1 receptor, on tissue sections and in cultured human primary retinal pigment epithelial and iris pigment epithelial (RPE/IPE) cell lines, using both qualitative and quantitative reverse transcription polymerase chain reaction (RT-PCR). Protein expression was studied using indirect immunofluorescence (IF).<br />Results: Almost all components of the classical RAS were found at high levels, at both the transcript and protein level, in the eyes' uvea and retina; and at lower levels in the cornea, conjunctiva and sclera. There was a much lower level of expression in the reference cultured RPE/IPE cells lines.<br />Conclusion: This study describes the distribution of RAS in the normal adult human eye and demonstrates the existence of an independent ocular RAS, with uveal and retinal tissues showing the highest expression of RAS components. These preliminary findings provide scope for examination of additional components of this system in the human eye, as well as possible differential expression under pathological conditions.<br /> (© The Author(s) 2014.)
- Subjects :
- Aged
Angiotensinogen biosynthesis
Cell Line
Female
Gene Expression physiology
Humans
Male
Middle Aged
Peptidyl-Dipeptidase A biosynthesis
Receptor, Angiotensin, Type 1 biosynthesis
Receptors, Cell Surface
Renin biosynthesis
Retina metabolism
Uvea metabolism
Prorenin Receptor
Eye metabolism
Renin-Angiotensin System physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1752-8976
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of the renin-angiotensin-aldosterone system : JRAAS
- Publication Type :
- Academic Journal
- Accession number :
- 25287897
- Full Text :
- https://doi.org/10.1177/1470320314549791