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A simple procedure to analyze positions of interest in infectious cell cultures by correlative light and electron microscopy.
- Source :
-
Methods in cell biology [Methods Cell Biol] 2014; Vol. 124, pp. 93-110. - Publication Year :
- 2014
-
Abstract
- Plastic cell culture dishes that contain a thin bottom of highest optical quality including an imprinted finder grid (μ-Dish Grid-500) are optimally suited for routine correlative light and electron microscopy using chemical fixation. Such dishes allow high-resolution fluorescence and bright-field imaging using fixed and living cells and are compatible with standard protocols for scanning and transmission electron microscopy. Ease of use during cell culture and imaging, as well as a tight cover render the dishes particularly suitable for working with infectious organisms up to the highest biosafety level. Detailed protocols are provided and demonstrated by showing two examples: monitoring the production of virus-like particles of the Human Endogenous Retrovirus HERV-K(HML-2) by HeLa cells and investigation of Rab11-positive membrane-compartments of HeLa cells after infection with Chlamydia trachomatis.<br /> (© 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Chlamydia trachomatis physiology
Cytoplasmic Vesicles metabolism
Cytoplasmic Vesicles ultrastructure
Endogenous Retroviruses physiology
HeLa Cells
Humans
Microscopy, Electron, Scanning methods
Microscopy, Electron, Transmission methods
Microscopy, Fluorescence methods
Microtomy
rab GTP-Binding Proteins metabolism
Single-Cell Analysis methods
Subjects
Details
- Language :
- English
- ISSN :
- 0091-679X
- Volume :
- 124
- Database :
- MEDLINE
- Journal :
- Methods in cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 25287838
- Full Text :
- https://doi.org/10.1016/B978-0-12-801075-4.00005-7