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OXA-23 carbapenemase in multidrug-resistant Acinetobacter baumannii ST2 type: first identification in L'Aquila Hospital (Italy).
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2015 Feb; Vol. 21 (1), pp. 97-101. Date of Electronic Publication: 2014 Oct 02. - Publication Year :
- 2015
-
Abstract
- In this study 114 extensively drug-resistant Acinetobacter baumannii clinical isolates were characterized. The strains were collected at L'Aquila Hospital after the earthquake in L'Aquila city (central Italy) on the 6th of April 2009. The genes blaOXA-23 and blaOXA-51 were detected in all clinical isolates analyzed, whereas blaTEM-1 allele was detected in 56/114 isolates. The blaOXA-23 gene is located downstream the ISAba region and is under control of a strong promoter. On 42/80 A. baumannii the presence of two class 1 integrons was ascertained on chromosomal DNA. Variable regions show different gene array: (1) aadB and aadA2, (2) aacA4, aac(6')-Ib-cr, and aadA1. Macrorestriction analysis using ApaI restriction endonuclease identifies three clusters (A, B, and C) according to pulsed-field gel electrophoresis profiles. All isolates analyzed belong to the clone A. baumannii sequence type 2.
- Subjects :
- Acinetobacter Infections epidemiology
Acinetobacter baumannii enzymology
Acinetobacter baumannii genetics
Base Sequence
Community-Acquired Infections epidemiology
Community-Acquired Infections microbiology
Conjugation, Genetic
DNA, Bacterial genetics
Electrophoresis, Gel, Pulsed-Field
Genes, Bacterial
Hospitals, Teaching statistics & numerical data
Humans
Italy epidemiology
Molecular Sequence Data
Multilocus Sequence Typing
Plasmids genetics
Polymorphism, Restriction Fragment Length
Acinetobacter Infections microbiology
Acinetobacter baumannii isolation & purification
Bacterial Proteins genetics
Drug Resistance, Multiple, Bacterial genetics
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 25275951
- Full Text :
- https://doi.org/10.1089/mdr.2014.0056