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N-WASP is required for Amphiphysin-2/BIN1-dependent nuclear positioning and triad organization in skeletal muscle and is involved in the pathophysiology of centronuclear myopathy.
- Source :
-
EMBO molecular medicine [EMBO Mol Med] 2014 Nov; Vol. 6 (11), pp. 1455-75. - Publication Year :
- 2014
-
Abstract
- Mutations in amphiphysin-2/BIN1, dynamin 2, and myotubularin are associated with centronuclear myopathy (CNM), a muscle disorder characterized by myofibers with atypical central nuclear positioning and abnormal triads. Mis-splicing of amphiphysin-2/BIN1 is also associated with myotonic dystrophy that shares histopathological hallmarks with CNM. How amphiphysin-2 orchestrates nuclear positioning and triad organization and how CNM-associated mutations lead to muscle dysfunction remains elusive. We find that N-WASP interacts with amphiphysin-2 in myofibers and that this interaction and N-WASP distribution are disrupted by amphiphysin-2 CNM mutations. We establish that N-WASP functions downstream of amphiphysin-2 to drive peripheral nuclear positioning and triad organization during myofiber formation. Peripheral nuclear positioning requires microtubule/Map7/Kif5b-dependent distribution of nuclei along the myofiber and is driven by actin and nesprins. In adult myofibers, N-WASP and amphiphysin-2 are only involved in the maintenance of triad organization but not in the maintenance of peripheral nuclear positioning. Importantly, we confirmed that N-WASP distribution is disrupted in CNM and myotonic dystrophy patients. Our results support a role for N-WASP in amphiphysin-2-dependent nuclear positioning and triad organization and in CNM and myotonic dystrophy pathophysiology.<br /> (© 2014 The Authors. Published under the terms of the CC BY 4.0 license.)
- Subjects :
- Adaptor Proteins, Signal Transducing genetics
Adult
Humans
Muscle Fibers, Skeletal metabolism
Mutant Proteins genetics
Mutant Proteins metabolism
Nuclear Proteins genetics
Tumor Suppressor Proteins genetics
Adaptor Proteins, Signal Transducing metabolism
Muscle, Skeletal physiopathology
Myopathies, Structural, Congenital physiopathology
Nuclear Proteins metabolism
Tumor Suppressor Proteins metabolism
Wiskott-Aldrich Syndrome Protein, Neuronal metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1757-4684
- Volume :
- 6
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- EMBO molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 25262827
- Full Text :
- https://doi.org/10.15252/emmm.201404436