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L-ferritin binding to scara5: a new iron traffic pathway potentially implicated in retinopathy.
- Source :
-
PloS one [PLoS One] 2014 Sep 26; Vol. 9 (9), pp. e106974. Date of Electronic Publication: 2014 Sep 26 (Print Publication: 2014). - Publication Year :
- 2014
-
Abstract
- Iron is essential in the retina because the heme-containing enzyme guanylate cyclase modulates phototransduction in rods and cones. Transferrin endocytosis is the classical pathway for obtaining iron from the blood circulation in the retina. However, the iron storage protein ferritin has been also recently proposed as an iron carrier. In this study, the presence of Scara5 and its binding to L-ferritin was investigated in the retina. Our results showed that Scara5, the specific receptor for L-ferritin, was expressed in mouse and human retinas in many cell types, including endothelial cells. Furthermore, we showed that intravenously injected ferritin crossed the blood retinal barrier through L-ferritin binding to Scara5 in endothelial cells. Thus, suggesting the existence of a new pathway for iron delivery and trafficking in the retina. In a murine model of photoreceptor degeneration, Scara5 was downregulated, pointing out this receptor as a potential player implicated in retinopathy and also as a possible therapeutic target.
- Subjects :
- Animals
Biological Transport
Disease Models, Animal
Endothelial Cells metabolism
Female
Ferritins blood
Ferritins genetics
Gene Expression
Gene Expression Regulation
Humans
Iron metabolism
Male
Mice
Photoreceptor Cells metabolism
Photoreceptor Cells pathology
Protein Binding
Protein Transport
Retina cytology
Retina metabolism
Retina pathology
Retinal Degeneration genetics
Retinal Degeneration metabolism
Retinal Degeneration pathology
Retinal Diseases metabolism
Retinal Diseases pathology
Retinal Neurons metabolism
Scavenger Receptors, Class A genetics
Ferritins metabolism
Scavenger Receptors, Class A metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 9
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 25259650
- Full Text :
- https://doi.org/10.1371/journal.pone.0106974