Study of the Stn protein in Salmonella; a regulator of membrane composition and integrity.

Authors :: Nakano M
Yamasaki E
Moss J
Hirayama T
Kurazono H
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2015; Vol. 1225, pp. 127-38.
Publication Year :: 2015
Abstract: Our studies were undertaken to develop new insights into the function of the Salmonella Stn protein. An analysis of total cell membrane protein fraction suggested the possibility that Stn associates with OmpA. This possibility was confirmed by immunogold labeling using anti-OmpA antibody and far-western blotting. From these results, we conclude that Stn regulates membrane composition and integrity in Salmonella.

Subjects :: Bacterial Outer Membrane Proteins metabolism
Blotting, Western
Enterotoxins isolation & purification
Immunohistochemistry
Microscopy, Electron
Recombinant Proteins isolation & purification
Recombinant Proteins metabolism
Salmonella ultrastructure
Cell Membrane chemistry
Cell Membrane metabolism
Enterotoxins metabolism
Salmonella cytology
Salmonella metabolism

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