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The use of three-dimensional printing to produce in vitro slice chambers.
- Source :
-
Journal of neuroscience methods [J Neurosci Methods] 2014 Dec 30; Vol. 238, pp. 82-7. Date of Electronic Publication: 2014 Sep 22. - Publication Year :
- 2014
-
Abstract
- Background: In recent years, 3D printing technology has become inexpensive and simple enough that any lab can own and use one of these printers.<br />New Method: We explored the potential use of 3D printers for quickly and easily producing in vitro slice chambers for patch clamp electrophysiology. Slice chambers were produced using five available plastics: ABS, PLA, Nylon 618, Nylon 680, and T-glase. These "lab-made" chambers were also made using stereolithography through a professional printing service (Shapeways). This study measured intrinsic membrane properties of neurons in the brain stem pedunculopontine nucleus (PPN) and layer V pyramidal neurons in retrosplenial cortex.<br />Results: Nylon 680 and T-glase significantly hyperpolarized PPN neurons. ABS increased input resistance, decreased action potential amplitude, and increased firing frequency in pyramidal cortical neurons. To test long term exposure to each plastic, human neuroblastoma SHSY5Y cell cultures were exposed to each plastic for 1 week. ABS decreased cell counts while Nylon 618 and Shapeways plastics eliminated cells. Primary mouse pituitary cultures were also tested for 24-h exposure. ABS decreased cell counts while Nylon 618 and Shapeways plastics dramatically decreased cell counts.<br />Comparison to Existing Methods: Chambers can be quickly and inexpensively printed in the lab. ABS, PLA, Nylon 680, and T-glase plastics would suffice for many experiments instead of commercially produced slice chambers.<br />Conclusions: While these technologies are still in their infancy, they represent a powerful addition to the lab environment. With careful selection of print material, slice chambers can be quickly and inexpensively manufactured in the lab.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Count
Cell Line, Tumor
Cell Survival drug effects
Cerebral Cortex drug effects
Cerebral Cortex physiology
Humans
Male
Materials Testing
Membrane Potentials drug effects
Mice
Neurons drug effects
Neurons physiology
Pedunculopontine Tegmental Nucleus drug effects
Pedunculopontine Tegmental Nucleus physiology
Pituitary Gland drug effects
Pituitary Gland physiology
Plastics toxicity
Rats, Sprague-Dawley
Patch-Clamp Techniques instrumentation
Plastics chemistry
Printing, Three-Dimensional
Tissue Culture Techniques instrumentation
Subjects
Details
- Language :
- English
- ISSN :
- 1872-678X
- Volume :
- 238
- Database :
- MEDLINE
- Journal :
- Journal of neuroscience methods
- Publication Type :
- Academic Journal
- Accession number :
- 25251556
- Full Text :
- https://doi.org/10.1016/j.jneumeth.2014.09.012