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Long-term vascular contractility assay using genipin-modified muscular thin films.
- Source :
-
Biofabrication [Biofabrication] 2014 Sep 23; Vol. 6 (4), pp. 045005. Date of Electronic Publication: 2014 Sep 23. - Publication Year :
- 2014
-
Abstract
- Vascular disease is a leading cause of death globally and typically manifests chronically due to long-term maladaptive arterial growth and remodeling. To date, there is no in vitro technique for studying vascular function over relevant disease time courses that both mimics in vivo-like tissue structure and provides a simple readout of tissue stress. We aimed to extend tissue viability in our muscular thin film contractility assay by modifying the polydimethylsiloxane (PDMS) substrate with micropatterned genipin, allowing extracellular matrix turnover without cell loss. To achieve this, we developed a microfluidic delivery system to pattern genipin and extracellular matrix proteins on PDMS prior to cell seeding. Tissues constructed using this method showed improved viability and maintenance of in vivo-like lamellar structure. Functional contractility of tissues fabricated on genipin-modified substrates remained consistent throughout two weeks in culture. These results suggest that muscular thin films with genipin-modified PDMS substrates are a viable method for conducting functional studies of arterial growth and remodeling in vascular diseases.
- Subjects :
- Cell Survival drug effects
Cells, Cultured
Dimethylpolysiloxanes chemistry
Fibronectins chemistry
Fibronectins pharmacology
Humans
Iridoids chemistry
Microfluidic Analytical Techniques methods
Muscle, Smooth, Vascular chemistry
Muscle, Smooth, Vascular drug effects
Tissue Engineering
Umbilical Arteries cytology
Bioprinting methods
Iridoids pharmacology
Microfluidic Analytical Techniques instrumentation
Models, Cardiovascular
Muscle, Smooth, Vascular cytology
Muscle, Smooth, Vascular physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1758-5090
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biofabrication
- Publication Type :
- Academic Journal
- Accession number :
- 25245868
- Full Text :
- https://doi.org/10.1088/1758-5082/6/4/045005