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FNR regulates expression of important virulence factors contributing to pathogenicity of uropathogenic Escherichia coli.
- Source :
-
Infection and immunity [Infect Immun] 2014 Dec; Vol. 82 (12), pp. 5086-98. Date of Electronic Publication: 2014 Sep 22. - Publication Year :
- 2014
-
Abstract
- Uropathogenic Escherichia coli (UPEC) is responsible for the majority of urinary tract infections (UTIs), which are some of the world's most common bacterial infections of humans. Here, we examined the role of FNR (fumarate and nitrate reduction), a well-known global regulator, in the pathogenesis of UPEC infections. We constructed an fnr deletion mutant of UPEC CFT073 and compared it to the wild type for changes in virulence, adherence, invasion, and expression of key virulence factors. Compared to the wild type, the fnr mutant was highly attenuated in the mouse model of human UTI and showed severe defects in adherence to and invasion of bladder and kidney epithelial cells. Our results showed that FNR regulates motility and multiple virulence factors, including expression of type I and P fimbriae, modulation of hemolysin expression, and expression of a novel pathogenicity island involved in α-ketoglutarate metabolism under anaerobic conditions. Our results demonstrate that FNR is a key global regulator of UPEC virulence and controls expression of important virulence factors that contribute to UPEC pathogenicity.<br /> (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)
- Subjects :
- Animals
Bacterial Adhesion
Disease Models, Animal
Epithelial Cells microbiology
Escherichia coli Infections microbiology
Escherichia coli Infections pathology
Escherichia coli Proteins genetics
Female
Gene Deletion
Iron-Sulfur Proteins genetics
Locomotion
Mice, Inbred CBA
Urinary Tract Infections microbiology
Urinary Tract Infections pathology
Uropathogenic Escherichia coli physiology
Virulence
Escherichia coli Proteins metabolism
Gene Expression Regulation, Bacterial
Iron-Sulfur Proteins metabolism
Uropathogenic Escherichia coli genetics
Uropathogenic Escherichia coli pathogenicity
Virulence Factors biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5522
- Volume :
- 82
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Infection and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 25245807
- Full Text :
- https://doi.org/10.1128/IAI.02315-14