Genotypic and phenotypic detection of efflux pump in Rhodococcus equi.

Authors :: Gressler LT
de Vargas AC
da Costa MM
Pötter L
da Silveira BP
Sangioni LA
de Avila Botton S
Source :: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2014 Aug 29; Vol. 45 (2), pp. 661-5. Date of Electronic Publication: 2014 Aug 29 (Print Publication: 2014).
Publication Year :: 2014
Abstract: The req&#95;39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.

Subjects :: Biological Transport, Active
DNA, Bacterial genetics
Ethidium metabolism
Polymerase Chain Reaction
Membrane Transport Proteins genetics
Membrane Transport Proteins metabolism
Rhodococcus equi genetics
Rhodococcus equi metabolism

Language :: English
ISSN :: 1678-4405
Volume :: 45
Issue :: 2
Database :: MEDLINE
Journal :: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Type :: Academic Journal
Accession number :: 25242956
Full Text :: https://doi.org/10.1590/s1517-83822014000200040