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Purinergic component in the coronary vasodilatation to acetylcholine after ischemia-reperfusion in perfused rat hearts.
- Source :
-
Journal of vascular research [J Vasc Res] 2014; Vol. 51 (4), pp. 283-9. Date of Electronic Publication: 2014 Sep 16. - Publication Year :
- 2014
-
Abstract
- To determine the involvement of purinergic receptors in coronary endothelium-dependent relaxation, the response to acetylcholine (1 × 10(-8) to 3 × 10(-7)M) was recorded in isolated rat hearts perfused according to the Langendorff procedure before and after 30 min of ischemia and 15 min of reperfusion and after the inhibition of nitric oxide synthesis with L-NAME (10(-4)M), in the absence and presence of the antagonist of purinergic P2X receptors, PPADS (3 × 10(-6)M), and of the antagonist of purinergic P2Y receptors, Reactive Blue 2 (3 × 10(-7)M). In control conditions, the relaxation to acetylcholine was not altered by PPADS or Reactive Blue 2. The relaxation to acetylcholine was reduced after ischemia-reperfusion, and, in this condition, it was further reduced by treatment with PPADS or Reactive Blue 2. Likewise, the relaxation to acetylcholine was reduced by L-NAME, and reduced further by Reactive Blue 2 but not by PPADS. These results suggest that the relaxation to acetylcholine may be partly mediated by purinergic receptors after ischemia-reperfusion, due to the reduction of nitric oxide release in this condition.<br /> (© 2014 S. Karger AG, Basel.)
- Subjects :
- Animals
Coronary Circulation physiology
Enzyme Inhibitors pharmacology
Heart drug effects
Male
Myocardial Reperfusion Injury physiopathology
NG-Nitroarginine Methyl Ester pharmacology
Nitroprusside pharmacology
Platelet Aggregation Inhibitors pharmacology
Purinergic P2X Receptor Antagonists pharmacology
Purinergic P2Y Receptor Antagonists pharmacology
Pyridoxal Phosphate analogs & derivatives
Pyridoxal Phosphate pharmacology
Rats, Sprague-Dawley
Triazines pharmacology
Vasodilation drug effects
Vasodilation physiology
Vasodilator Agents pharmacology
Acetylcholine pharmacology
Coronary Circulation drug effects
Heart physiology
Myocardial Reperfusion Injury drug therapy
Receptors, Purinergic P2X physiology
Receptors, Purinergic P2Y physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0135
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of vascular research
- Publication Type :
- Academic Journal
- Accession number :
- 25228127
- Full Text :
- https://doi.org/10.1159/000365928