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Pediatric microdose study of [(14)C]paracetamol to study drug metabolism using accelerated mass spectrometry: proof of concept.
- Source :
-
Clinical pharmacokinetics [Clin Pharmacokinet] 2014 Nov; Vol. 53 (11), pp. 1045-51. - Publication Year :
- 2014
-
Abstract
- Background: Pediatric drug development is hampered by practical, ethical, and scientific challenges. Microdosing is a promising new method to obtain pharmacokinetic data in children with minimal burden and minimal risk. The use of a labeled oral microdose offers the added benefit to study intestinal and hepatic drug disposition in children already receiving an intravenous therapeutic drug dose for clinical reasons.<br />Objective: The objective of this study was to present pilot data of an oral [(14)C]paracetamol [acetaminophen (AAP)] microdosing study as proof of concept to study developmental pharmacokinetics in children.<br />Methods: In an open-label microdose pharmacokinetic pilot study, infants (0-6 years of age) received a single oral [(14)C]AAP microdose (3.3 ng/kg, 60 Bq/kg) in addition to intravenous therapeutic doses of AAP (15 mg/kg intravenous every 6 h). Blood samples were taken from an indwelling catheter. AAP blood concentrations were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and [(14)C]AAP and metabolites ([(14)C]AAP-Glu and [(14)C]AAP-4Sul) were measured by accelerator mass spectrometry.<br />Results: Ten infants (aged 0.1-83.1 months) were included; one was excluded as he vomited shortly after administration. In nine patients, [(14)C]AAP and metabolites in blood samples were detectable at expected concentrations: median (range) maximum concentration (C max) [(14)C]AAP 1.68 (0.75-4.76) ng/L, [(14)C]AAP-Glu 0.88 (0.34-1.55) ng/L, and [(14)C]AAP-4Sul 0.81 (0.29-2.10) ng/L. Dose-normalized oral [(14)C]AAP C max approached median intravenous average concentrations (C av): 8.41 mg/L (3.75-23.78 mg/L) and 8.87 mg/L (3.45-12.9 mg/L), respectively.<br />Conclusions: We demonstrate the feasibility of using a [(14)C]labeled microdose to study AAP pharmacokinetics, including metabolite disposition, in young children.
- Subjects :
- Acetaminophen chemistry
Administration, Intravenous
Administration, Oral
Analgesics, Non-Narcotic chemistry
Carbon Radioisotopes
Child
Child, Preschool
Chromatography, Liquid methods
Drug Administration Schedule
Feasibility Studies
Female
Humans
Infant
Infant, Newborn
Male
Netherlands
Pilot Projects
Acetaminophen administration & dosage
Acetaminophen pharmacokinetics
Analgesics, Non-Narcotic administration & dosage
Analgesics, Non-Narcotic pharmacokinetics
Tandem Mass Spectrometry methods
Subjects
Details
- Language :
- English
- ISSN :
- 1179-1926
- Volume :
- 53
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Clinical pharmacokinetics
- Publication Type :
- Academic Journal
- Accession number :
- 25227283
- Full Text :
- https://doi.org/10.1007/s40262-014-0176-8