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A diagnostic model for histologic damage in undescended testes based on testis rigidity measurement: an experimental study with a novel device.
- Source :
-
The Journal of surgical research [J Surg Res] 2014 Dec; Vol. 192 (2), pp. 521-30. Date of Electronic Publication: 2014 Jul 24. - Publication Year :
- 2014
-
Abstract
- Background: We aimed to test whether testis rigidity (hardness) measured using a newly-designed device we previously introduced would offer more reliable assessment of histologic damage in undescended testes than conventional methods (consistency feel at palpation, volume measurement).<br />Materials and Methods: Forty-five 18-d-old Lewis rats underwent surgical inhibition of descent of left testes and were followed to 40 (n = 16), 63 (n = 14), or 90 days (n = 15). Another 45 18-d-old Lewis rats were sham operated (left side) and followed likewise (n = 14, n = 15, and n = 16). At the designated time points, testes were exposed bilaterally, rigidity was measured, and consistency at palpation was scored; testes were removed and subjected to length, width, weight measurements, volume calculation, and histomorphometry (mean Johnsen score [MJS], mean tubular diameter [MTD], and mean capsule width [MCW]). Testes of experimental group were compared with ipsilateral testes of sham-operated rats.<br />Results: At all time points, undescended testes had decreased rigidity, MJS, and MTD, increased MCW, decreased volume and weight; contralateral testes remained unaffected. Rigidity was associated only with MJS and MTD, and most strongly with MJS (multiple stepwise linear regression, F = 694.44, P < 0.0005). MJS could be precisely predicted from rigidity: MJS = 0.699 × testis rigidity (F = 1358.82, P < 0.0005). This model showed good fit between predicted and actual MJS values (R(2) = 0.94), low error, nonsignificant bias, sensitivity 75% and specificity 90%. Model validation showed low prediction error and nonsignificant bias, indicating generalizability. Testis volume and palpation proved imprecise MJS predictors.<br />Conclusions: Testis rigidity is an effective predictor of histologic damage in rat undescended testes, with diagnostic value superior to testis palpation scoring and volume measurement.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Biopsy
Calibration
Elasticity
Equipment Design
Hardness Tests instrumentation
Male
Palpation
Predictive Value of Tests
Rats, Inbred Lew
Spermatids cytology
Spermatocytes cytology
Spermatogenesis
Torque
Cryptorchidism diagnosis
Cryptorchidism pathology
Cryptorchidism physiopathology
Hardness Tests methods
Testis pathology
Testis physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8673
- Volume :
- 192
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- The Journal of surgical research
- Publication Type :
- Academic Journal
- Accession number :
- 25214261
- Full Text :
- https://doi.org/10.1016/j.jss.2014.07.032