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Fifteen novel immunoreactive proteins of Chinese virulent Haemophilus parasuis serotype 5 verified by an immunoproteomic assay.
- Source :
-
Folia microbiologica [Folia Microbiol (Praha)] 2015 Jan; Vol. 60 (1), pp. 81-7. Date of Electronic Publication: 2014 Sep 09. - Publication Year :
- 2015
-
Abstract
- Haemophilus parasuis (H. parasuis) is associated with meningitis, polyserositis, polyarthritis and bacterial pneumonia. At present, its prevention and control is difficult because of the lack of suitable subunit vaccines. Nowadays, high-throughput methods, immunoproteomics, are available to screen for more vaccine candidates. A protein extraction method for H. parasuis and two-dimensional electrophoresis (2-DE) were optimized to provide high-resolution profiles covering pH 3 to 10. Twenty immunoreactive spots were excised from gels after strict comparison between 2-DE Western blot membranes and the relevant gels. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and MALDI-TOF-TOF-MS successfully identified 16 different proteins. Fifteen of them were reported as immunoreactive proteins in H. parasuis for the first time. In addition, recombinant HP5-7 (ABC transporter, periplasmic-binding protein) showed immunoreactivity both with hyperimmune rabbit serum and convalescent swine serum. Four recombinants of the 14 successfully expressed genes showed immunoreactivity with hyperimmune rabbit serum.
- Subjects :
- Animals
Antibodies, Bacterial analysis
Antibodies, Bacterial immunology
Bacterial Proteins genetics
Bacterial Proteins immunology
Blotting, Western
China
Electrophoresis, Gel, Two-Dimensional
Haemophilus Infections immunology
Haemophilus Infections microbiology
Haemophilus parasuis classification
Haemophilus parasuis genetics
Haemophilus parasuis immunology
Mass Spectrometry
Molecular Sequence Data
Proteomics
Rabbits
Serogroup
Swine
Swine Diseases immunology
Swine Diseases microbiology
Bacterial Proteins chemistry
Haemophilus Infections veterinary
Haemophilus parasuis chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1874-9356
- Volume :
- 60
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Folia microbiologica
- Publication Type :
- Academic Journal
- Accession number :
- 25200063
- Full Text :
- https://doi.org/10.1007/s12223-014-0343-1