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CHD7 maintains neural stem cell quiescence and prevents premature stem cell depletion in the adult hippocampus.
- Source :
-
Stem cells (Dayton, Ohio) [Stem Cells] 2015 Jan; Vol. 33 (1), pp. 196-210. - Publication Year :
- 2015
-
Abstract
- Neural stem/progenitor cells (NSCs) in the hippocampus produce new neurons throughout adult life. NSCs are maintained in a state of reversible quiescence and the failure to maintain the quiescent state can result in the premature depletion of the stem cell pool. The epigenetic mechanisms that maintain this quiescent state have not been identified. Using an inducible knockout mouse model, we show that the chromatin remodeling factor chromodomain-helicase-DNA-binding protein 7 (CHD7) is essential for maintaining NSC quiescence. CHD7 inactivation in adult NSCs results in a loss of stem cell quiescence in the hippocampus, a transient increase in cell divisions, followed by a significant decline in neurogenesis. This loss of NSC quiescence is associated with the premature loss of NSCs in middle-aged mice. We find that CHD7 represses the transcription of several positive regulators of cell cycle progression and is required for full induction of the Notch target gene Hes5 in quiescent NSCs. These findings directly link CHD7 to pathways involved in NSC quiescence and identify the first chromatin-remodeling factor with a role in NSC quiescence and maintenance. As CHD7 haplo-insufficiency is associated with a range of cognitive disabilities in CHARGE syndrome, our observations may have implications for understanding the basis of these deficits.<br /> (© 2014 AlphaMed Press.)
- Subjects :
- Animals
Cell Differentiation physiology
Cell Proliferation physiology
DNA Helicases biosynthesis
DNA Helicases genetics
DNA-Binding Proteins genetics
Gene Expression Regulation
Hippocampus metabolism
Humans
Mice
Neural Stem Cells metabolism
Neurogenesis physiology
DNA-Binding Proteins biosynthesis
Hippocampus cytology
Neural Stem Cells cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1549-4918
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Stem cells (Dayton, Ohio)
- Publication Type :
- Academic Journal
- Accession number :
- 25183173
- Full Text :
- https://doi.org/10.1002/stem.1822