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Purification and characterization of high antioxidant peptides from duck egg white protein hydrolysates.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 Oct 03; Vol. 452 (4), pp. 888-94. Date of Electronic Publication: 2014 Aug 30. - Publication Year :
- 2014
-
Abstract
- The hydrolysate from duck egg white protein (DEWP) prepared by "SEEP-Alcalase" at degree of hydrolysis (DH) value of 21% (namely HSA21) exhibited high antioxidant capacity in different oxidation systems. A consecutive chromatographic method was then developed for separation and purification of HSA21, including ion-exchange chromatography, macroporous adsorption resin (MAR) and gel filter chromatography. The final peptides "P21-3-75-B" were obtained with significantly enhanced antioxidant activity (p<0.05). It was further confirmed that the product mainly consisted of five oligopeptides (Mr: 202.1, 294.1, 382.1, 426.3, and 514.4Da). Furthermore, the antioxidant activity of P21-3-75-B kept stable after in vitro digestive simulation. Antioxidant capacity of the purified peptides was closely related to the molecular mass, hydrophobic amino acid residues, acidic amino acid and some antioxidant amino acids. This research provided a valuable route for producing new natural-source peptides with strong antioxidant capacity and high nutritious value for our daily intake.<br /> (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Drug Stability
Free Radicals chemistry
Gastrointestinal Contents chemistry
Hydrolysis
Oxygen chemistry
Protein Hydrolysates chemistry
Protein Hydrolysates isolation & purification
Antioxidants chemistry
Antioxidants isolation & purification
Ducks metabolism
Egg Proteins chemistry
Egg Proteins isolation & purification
Oligopeptides chemistry
Oligopeptides isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2104
- Volume :
- 452
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 25181341
- Full Text :
- https://doi.org/10.1016/j.bbrc.2014.08.116