Back to Search
Start Over
Ubiquitin-like protein MNSFβ negatively regulates T cell function and survival.
- Source :
-
Immunological investigations [Immunol Invest] 2015; Vol. 44 (1), pp. 1-12. Date of Electronic Publication: 2014 Sep 02. - Publication Year :
- 2015
-
Abstract
- Monoclonal non-specific suppressor factor β (MNSFβ) is a ubiquitously expressed member of the ubiquitin-like family that is involved in various biological functions. Previous studies have demonstrated that MNSFβ covalently binds to intracellular pro-apoptotic protein Bcl-G and regulates apoptosis in macrophages. In this study, we demonstrate that MNSFβ negatively regulates T cell function. In murine T-helper type 2 clone, D10.G4.1 (D10) cells transfected with MNSFβ cDNA, CD3/CD28-induced ERK1/2 phosphorylation leading to IL-4 production was significantly inhibited. The formation of MNSFβ-Bcl-G complex was induced by the CD3/CD28 stimulation. Co-transfection with MNSFβ and Bcl-G greatly enhanced CD3/CD28-induced apoptosis in D10 cells. Similarly, co-over-expression of MNSFβ and Bcl-G caused a marked enhancement of apoptosis in purified splenic T cells. Interestingly, this MNSFβ adduct was also induced in T cells derived from DO11.10 mice stimulated with antigen. Collectively, CD3/CD28-inducible MNSFβ-Bcl-G complex may be involved in the regulation of T cell function and survival.
- Subjects :
- Animals
Antibodies, Monoclonal pharmacology
Apoptosis
B-Lymphocytes cytology
B-Lymphocytes drug effects
B-Lymphocytes metabolism
CD28 Antigens metabolism
CD3 Complex metabolism
Cell Line
Cell Proliferation
Cell Survival
Interleukin-4 biosynthesis
Interleukin-4 metabolism
Mice
Mice, Inbred BALB C
Mitogen-Activated Protein Kinase 1 genetics
Mitogen-Activated Protein Kinase 1 metabolism
Mitogen-Activated Protein Kinase 3 genetics
Mitogen-Activated Protein Kinase 3 metabolism
Primary Cell Culture
Protein Binding
Proto-Oncogene Proteins c-bcl-2 metabolism
Signal Transduction
Spleen cytology
Spleen drug effects
Spleen metabolism
Suppressor Factors, Immunologic metabolism
T-Lymphocytes, Helper-Inducer cytology
T-Lymphocytes, Helper-Inducer drug effects
Transfection
CD28 Antigens genetics
CD3 Complex genetics
Gene Expression Regulation
Proto-Oncogene Proteins c-bcl-2 genetics
Suppressor Factors, Immunologic genetics
T-Lymphocytes, Helper-Inducer metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4311
- Volume :
- 44
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 25180634
- Full Text :
- https://doi.org/10.3109/08820139.2014.909454