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Chemical biology of genomic DNA: minimizing PCR bias.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2014 Oct 18; Vol. 50 (81), pp. 12047-9. - Publication Year :
- 2014
-
Abstract
- The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 50
- Issue :
- 81
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 25178041
- Full Text :
- https://doi.org/10.1039/c4cc05107f