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X-ray nanoprobes and diffraction-limited storage rings: opportunities and challenges of fluorescence tomography of biological specimens.
- Source :
-
Journal of synchrotron radiation [J Synchrotron Radiat] 2014 Sep; Vol. 21 (Pt 5), pp. 1031-47. Date of Electronic Publication: 2014 Aug 27. - Publication Year :
- 2014
-
Abstract
- X-ray nanoprobes require coherent illumination to achieve optic-limited resolution, and so will benefit directly from diffraction-limited storage rings. Here, the example of high-resolution X-ray fluorescence tomography is focused on as one of the most voracious demanders of coherent photons, since the detected signal is only a small fraction of the incident flux. Alternative schemes are considered for beam delivery, sample scanning and detectors. One must consider as well the steps before and after the X-ray experiment: sample preparation and examination conditions, and analysis complexity due to minimum dose requirements and self-absorption. By understanding the requirements and opportunities for nanoscale fluorescence tomography, one gains insight into the R&D challenges in optics and instrumentation needed to fully exploit the source advances that diffraction-limited storage rings offer.
- Subjects :
- Image Enhancement instrumentation
Image Enhancement methods
Nanoparticles
Optics and Photonics
Radiation Dosage
Synchrotrons
X-Ray Diffraction instrumentation
X-Ray Microtomography instrumentation
Microscopy, Fluorescence
Molecular Probe Techniques instrumentation
X-Ray Diffraction methods
X-Ray Microtomography methods
Subjects
Details
- Language :
- English
- ISSN :
- 1600-5775
- Volume :
- 21
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- Journal of synchrotron radiation
- Publication Type :
- Academic Journal
- Accession number :
- 25177992
- Full Text :
- https://doi.org/10.1107/S160057751401621X