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In vitro evaluation of Cordyceps militaris as a potential radioprotective agent.
- Source :
-
International journal of molecular medicine [Int J Mol Med] 2014 Nov; Vol. 34 (5), pp. 1349-57. Date of Electronic Publication: 2014 Aug 18. - Publication Year :
- 2014
-
Abstract
- Radiation is an important component of therapy for a wide range of malignant conditions. However, it triggers DNA damage and cell death in normal cells and results in adverse side-effects. Cordyceps militaris (C. militaris), a traditional medicinal mushroom, produces the bioactive compound, cordycepin (3'-deoxyadenosine) and has multiple pharmacological activities, such as antitumor, antimetastatic, antioxidant and immunomodulatory effects. The present study was undertaken to investigate whether CM-AE, an extract obtained from C. militaris exerts protective effects against radiation-induced DNA damage. The protective effects of CM-AE were compared with those of cordycepin. CM-AE effectively increased free radical scavenging activity and decreased radiation-induced plasmid DNA strand breaks in in vitro assays. CM-AE significantly inhibited the generation of reactive oxygen species (ROS) and cellular DNA damage in 2 Gy irradiated Chinese hamster ovary (CHO)-K1 cells. Moreover, treatment with CM-AE induced similar levels of phosphorylated H2AX in the cells, which reflects the initial DNA double-strand breaks in the irradiated cells compared with the non-irradiated CHO-K1 cells. However, cordycepin did not show free radical scavenging activity and did not protect against radiation-induced plasmid DNA or cellular DNA damage. These results suggest that the free radical scavenging activity of CM-AE contributes towards its DNA radioprotective effects and that the protective effects of CM-AE are much more potent to those of cordycepin. The data presented in this study may provide useful information for the screening of potent radioprotective materials.
- Subjects :
- Agaricales chemistry
Animals
CHO Cells
Cell Survival drug effects
Comet Assay
Cricetinae
Cricetulus
DNA Damage drug effects
DNA Damage radiation effects
Deoxyadenosines pharmacology
Dose-Response Relationship, Radiation
Gamma Rays adverse effects
Phosphorylation
Reactive Oxygen Species metabolism
Cordyceps chemistry
Radiation-Protective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-244X
- Volume :
- 34
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 25176413
- Full Text :
- https://doi.org/10.3892/ijmm.2014.1901