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Monitoring stroke progression: in vivo imaging of cortical perfusion, blood-brain barrier permeability and cellular damage in the rat photothrombosis model.
- Source :
-
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2014 Nov; Vol. 34 (11), pp. 1791-801. Date of Electronic Publication: 2014 Aug 27. - Publication Year :
- 2014
-
Abstract
- Focal cerebral ischemia is among the main causes of death and disability worldwide. The ischemic core often progresses, invading the peri-ischemic brain; however, assessing the propensity of the peri-ischemic brain to undergo secondary damage, understanding the underlying mechanisms, and adjusting treatment accordingly remain clinically unmet challenges. A significant hallmark of the peri-ischemic brain is dysfunction of the blood-brain barrier (BBB), yet the role of disturbed vascular permeability in stroke progression is unclear. Here we describe a longitudinal in vivo fluorescence imaging approach for the evaluation of cortical perfusion, BBB dysfunction, free radical formation and cellular injury using the photothrombosis vascular occlusion model in male Sprague Dawley rats. Blood-brain barrier dysfunction propagated within the peri-ischemic brain in the first hours after photothrombosis and was associated with free radical formation and cellular injury. Inhibiting free radical signaling significantly reduced progressive cellular damage after photothrombosis, with no significant effect on blood flow and BBB permeability. Our approach allows a dynamic follow-up of cellular events and their response to therapeutics in the acutely injured cerebral cortex.
- Subjects :
- Animals
Disease Models, Animal
Free Radicals metabolism
Male
Permeability
Rats
Rats, Sprague-Dawley
Blood-Brain Barrier metabolism
Blood-Brain Barrier pathology
Blood-Brain Barrier physiopathology
Brain Ischemia metabolism
Brain Ischemia pathology
Brain Ischemia physiopathology
Cerebral Cortex metabolism
Cerebral Cortex pathology
Cerebral Cortex physiopathology
Cerebrovascular Circulation
Intracranial Thrombosis metabolism
Intracranial Thrombosis pathology
Intracranial Thrombosis physiopathology
Stroke metabolism
Stroke pathology
Stroke physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1559-7016
- Volume :
- 34
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 25160672
- Full Text :
- https://doi.org/10.1038/jcbfm.2014.147