Back to Search
Start Over
Quantification of human mAbs in mouse tissues using generic affinity enrichment procedures and LC-MS detection.
- Source :
-
Bioanalysis [Bioanalysis] 2014; Vol. 6 (13), pp. 1795-811. - Publication Year :
- 2014
-
Abstract
- Background: The disease state can modulate the penetration of large antibody-sized therapeutic molecules into affected tissues. Suitable bioanalytical methods are required for the quantitative analysis of drug tissue levels to enable a better understanding of the parameters influencing drug penetration and target engagement.<br />Results: Described is a sensitive and selective LC-MS/MS assay for the quantification of human mAb molecules in mouse tissues. By homogenizing tissues directly into serum, a common serum calibration curve can be used for multiple tissues. A generic procedure was used for affinity enrichment. An analytical range of 20 - 20,000 ng/ml was achieved in serum.<br />Conclusion: The method described here can be applied for the quantitative analysis of mAb and Fc-fusion therapeutic molecules in a variety of animal tissue matrices.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies, Monoclonal blood
Antibodies, Monoclonal metabolism
Antibodies, Monoclonal, Humanized analysis
Antibodies, Monoclonal, Humanized blood
Antibodies, Monoclonal, Humanized metabolism
Chromatography, Affinity
Female
Humans
Liver metabolism
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Peptides analysis
Regression Analysis
Skin metabolism
Trypsin metabolism
Ustekinumab
Antibodies, Monoclonal analysis
Chromatography, High Pressure Liquid
Tandem Mass Spectrometry
Subjects
Details
- Language :
- English
- ISSN :
- 1757-6199
- Volume :
- 6
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Bioanalysis
- Publication Type :
- Academic Journal
- Accession number :
- 25157486
- Full Text :
- https://doi.org/10.4155/bio.14.143