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Intracellular staining for cytokines and transcription factors.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1193, pp. 39-49. - Publication Year :
- 2014
-
Abstract
- Within the past years immune cells in general and T cells in particular have been categorized into a vast variety of subsets with different functional properties. One of the key technologies fueling this emerging complexity is intracellular staining for effector cytokines and/or lineage-defining transcription factors. Here we discuss the critical steps for performing successful multicolor immunophenotyping of mouse T cells in combination with analysis of intracellular molecules after ex vivo isolation.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1193
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 25150995
- Full Text :
- https://doi.org/10.1007/978-1-4939-1212-4_5