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Anti-trichophyton activity of protocatechuates and their synergism with fluconazole.

Authors :
Soares LA
Gullo FP
Sardi Jde C
Pitangui Nde S
Costa-Orlandi CB
Sangalli-Leite F
Scorzoni L
Regasini LO
Petrônio MS
Souza PF
Silva DH
Mendes-Giannini MJ
Fusco-Almeida AM
Source :
Evidence-based complementary and alternative medicine : eCAM [Evid Based Complement Alternat Med] 2014; Vol. 2014, pp. 957860. Date of Electronic Publication: 2014 Jun 18.
Publication Year :
2014

Abstract

Dermatophytosis and superficial mycosis are a major global public health problem that affects 20-25% of the world's population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents due to their broad spectrum of biological activities. This study aimed to verify the antifungal activity of protocatechuic acid, 3,4-diacetoxybenzoic, and fourteen alkyl protocatechuates (3,4-dihydroxybenzoates) against Trichophyton rubrum and Trichophyton mentagrophytes and to further assess their activities when combined with fluconazole. Susceptibility and synergism assays were conducted as described in M38-A2 (CLSI), with modifications. Three strains of Trichophyton rubrum and three strains of Trichophyton mentagrophytes were used in this work. The pentyl, hexyl, heptyl, octyl, nonyl, and decyl protocatechuates showed great fungicidal effects, with minimum inhibitory concentration (MIC) values ranging from 0.97 to 7.8 mg/L. Heptyl showed a synergistic activity (FIC index = 0.49), reducing the MIC of fluconazole by fourfold. All substances tested were safe, especially the hexyl, heptyl, octyl, and nonyl compounds, all of which showed a high selectivity index, particularly in combination with fluconazole. These ester associations with fluconazole may represent a promising source of prototypes in the search for anti-Trichophyton therapeutic agents.

Details

Language :
English
ISSN :
1741-427X
Volume :
2014
Database :
MEDLINE
Journal :
Evidence-based complementary and alternative medicine : eCAM
Publication Type :
Academic Journal
Accession number :
25136374
Full Text :
https://doi.org/10.1155/2014/957860