Characterization of a novel AmpC β-lactamase produced by a carbapenem-resistant Cedecea davisae clinical isolate.

Authors :: Ammenouche N
Dupont H
Mammeri H
Source :: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2014 Nov; Vol. 58 (11), pp. 6942-5. Date of Electronic Publication: 2014 Aug 18.
Publication Year :: 2014
Abstract: A Cedecea davisae isolate, which was intermediate or resistant to third-generation cephalosporins and carbapenems, was recovered from a urine sample. Susceptibility testing, isoelectric focusing, and analysis of outer membrane proteins showed that AmpC β-lactamase expression combined with porin deficiency accounted for the carbapenem resistance. A cloning experiment followed by phenotypic and enzymatic characterization identified a novel class C enzyme that was phylogenetically and biochemically close to the chromosome-borne β-lactamases of the genera Enterobacter and Citrobacter.<br /> (Copyright © 2014, American Society for Microbiology. All Rights Reserved.)

Subjects :: Aged
Amino Acid Sequence
Bacterial Outer Membrane Proteins genetics
Bacterial Proteins biosynthesis
Drug Resistance, Bacterial genetics
Enterobacteriaceae genetics
Female
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Phylogeny
Porins genetics
beta-Lactamases biosynthesis
Bacterial Proteins genetics
Carbapenems pharmacology
Enterobacteriaceae drug effects
Enterobacteriaceae enzymology
beta-Lactamases genetics

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