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Extrachromosomal circular DNAs in Drosophila melanogaster: comparison between embryos and Kc0% cells.
- Source :
-
Chromosoma [Chromosoma] 1989 Sep; Vol. 98 (3), pp. 201-6. - Publication Year :
- 1989
-
Abstract
- We established the size distribution of extrachromosomal covalently closed circular DNA molecules from embryos of various Drosophila melanogaster strains and from Kc0% tissue culture cells. In embryos, more than 80% of the circular DNA molecules are smaller than 2.5 kb and all the distributions show a peak of molecules of between 200 and 400 bp. The Kc0% cell distribution differs mainly from that of embryos in that 48% of the molecules have a size between 4 and 8 kb. Correlating with this, circular molecules homologous to copia, 412 and 297 were detected only in Kc0% cells. The three tandemly repeated families containing the 5S genes, the histone genes and the 240 bp repeat of the ribosomal DNA intergenic spacer, which had previously been identified in circular DNAs from embryos, were also found in cultured cells. A fourth tandemly repeated family corresponding to the 1.688 g/cm3 satellite DNA was detected, both in embryos and Kc0% cells. It consists of circular multimeric molecules containing multiple copies of the 359 bp repeated unit. No circular DNA molecules homologous to the actin genes, the type I ribosomal DNA insertion, or the F and I transposable elements were found in embryos or Kc0% cells. Thus it appears that the extrachromosomal circular DNA molecules from embryos and from tissue culture cells differ mainly in the presence of circular copies of the copia-like transposable elements.
- Subjects :
- Animals
DNA, Circular ultrastructure
DNA, Satellite analysis
DNA, Satellite ultrastructure
Embryo, Nonmammalian chemistry
Embryo, Nonmammalian ultrastructure
Female
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Base Sequence
DNA, Circular analysis
Drosophila melanogaster genetics
Extrachromosomal Inheritance
Sequence Homology, Nucleic Acid
Subjects
Details
- Language :
- English
- ISSN :
- 0009-5915
- Volume :
- 98
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Chromosoma
- Publication Type :
- Academic Journal
- Accession number :
- 2510979
- Full Text :
- https://doi.org/10.1007/BF00329684