Antigenicity and immunogenicity of fused B-subunit of heat labile toxin of Escherichia coli and colonization factor antigen I polyepitopes.

Authors :: Savar NS
Dashti A
Darzi Eslam E
Jahanian-Najafabadi A
Jafari A
Source :: Journal of microbiological methods [J Microbiol Methods] 2014 Nov; Vol. 106, pp. 40-46. Date of Electronic Publication: 2014 Aug 07.
Publication Year :: 2014
Abstract: Linear B-cell epitopes ((93)AKEFEAAAL(101) and (66)PQLTDVLN(73)) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)

Subjects :: Animals
Bacterial Toxins genetics
Enterotoxins genetics
Epitopes genetics
Escherichia coli genetics
Escherichia coli Proteins genetics
Fimbriae Proteins genetics
Hemagglutination Tests
Rabbits
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Antibodies, Bacterial immunology
Bacterial Toxins immunology
Enterotoxins immunology
Epitopes immunology
Escherichia coli immunology
Escherichia coli Proteins immunology
Fimbriae Proteins immunology

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