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Capsid-Damaging Effects of UV Irradiation as Measured by Quantitative PCR Coupled with Ethidium Monoazide Treatment.
- Source :
-
Food and environmental virology [Food Environ Virol] 2014 Dec; Vol. 6 (4), pp. 269-75. Date of Electronic Publication: 2014 Aug 09. - Publication Year :
- 2014
-
Abstract
- The damage to a viral capsid after low-pressure (LP) and medium-pressure (MP) UV irradiation was assessed, using the quantitative or quantitative reverse transcription PCR coupled with ethidium monoazide treatment (EMA-PCR). After UV irradiation, adenovirus 5 (Ad5) and poliovirus 1 (PV1) were subjected to a plaque assay, PCR, and EMA-PCR to investigate the effect of UV irradiation on viral infectivity, genome damage, and capsid damage, respectively. The effectiveness of UV wavelengths in a viral genome and capsid damage of both PV1 and Ad5 was also further investigated using a band-pass filter. It was found that an MPUV lamp was more effective than an LPUV lamp in inactivating Ad5, whereas there was no difference in the case of PV1. The results of viral reduction determined by PCR and EMA-PCR indicated that MP UV irradiation damaged Ad5 capsid. The damage to PV1 and Ad5 capsid was also not observed after LP UV irradiation. The investigation of effects of UV wavelengths suggested that UV wavelengths at 230-245 nm have greater effects on adenovirus capsid in addition to viral genome than UV wavelengths beyond 245 nm.
- Subjects :
- Adenoviruses, Human growth & development
Adenoviruses, Human metabolism
Adenoviruses, Human pathogenicity
Animals
Capsid metabolism
Cell Line
Chlorocebus aethiops
DNA, Viral metabolism
DNA, Viral radiation effects
Humans
Poliovirus growth & development
Poliovirus metabolism
Poliovirus pathogenicity
Pressure
RNA, Viral metabolism
RNA, Viral radiation effects
Radiation Tolerance
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Ultraviolet Rays
Viral Plaque Assay
Virus Inactivation radiation effects
Adenoviruses, Human radiation effects
Affinity Labels pharmacology
Azides pharmacology
Capsid radiation effects
Disinfection methods
Genome, Viral radiation effects
Poliovirus radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1867-0342
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Food and environmental virology
- Publication Type :
- Academic Journal
- Accession number :
- 25106777
- Full Text :
- https://doi.org/10.1007/s12560-014-9162-4