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Anesthetic preconditioning inhibits isoflurane-mediated apoptosis in the developing rat brain.
- Source :
-
Anesthesia and analgesia [Anesth Analg] 2014 Oct; Vol. 119 (4), pp. 939-946. - Publication Year :
- 2014
-
Abstract
- Background: We hypothesized that preconditioning (PC) with a short exposure to isoflurane (ISO) would reduce neurodegeneration induced by prolonged exposure to ISO in neonatal rats, as previously shown in neuronal cell culture.<br />Methods: We randomly divided 7-day-old Sprague-Dawley rats into 3 groups: control, 1.5% ISO, and PC + 1.5% ISO. The control group was exposed to carrier gas (30% oxygen balanced in nitrogen) for 30 minutes and then to carrier gas again for 6 hours the following day. The 1.5% ISO group was exposed to carrier gas for 30 minutes and then to 1.5% ISO for 6 hours the following day. The PC + 1.5% ISO group was preconditioned with a 30-minute 1.5% ISO exposure and then exposed to 1.5% ISO for 6 hours the following day. Blood and brain samples were collected 2 hours after the exposures for determination of neurodegenerative biomarkers, including caspase-3, S100β, caspase-12, and an autophagy biomarker Beclin-1.<br />Results: Prolonged exposure to ISO significantly increased cleaved caspase-3 expression in the cerebral cortex of 7-day-old rats compared with the group preconditioned with ISO and the controls using Western blot assays. However, significant differences were not detected for other markers of neuronal injury.<br />Conclusions: The ISO-mediated increase in cleaved caspase-3 in the postnatal day 7 rat brain is ameliorated by PC with a brief anesthetic exposure, and differences were not detected in other markers of neuronal injury.
- Subjects :
- Anesthetics, Inhalation adverse effects
Animals
Animals, Newborn
Apoptosis physiology
Female
Isoflurane adverse effects
Male
Random Allocation
Rats
Rats, Sprague-Dawley
Anesthetics, Inhalation administration & dosage
Apoptosis drug effects
Brain drug effects
Brain growth & development
Isoflurane administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 1526-7598
- Volume :
- 119
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Anesthesia and analgesia
- Publication Type :
- Academic Journal
- Accession number :
- 25099925
- Full Text :
- https://doi.org/10.1213/ANE.0000000000000380