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Retrospective case-control study of viral pathogen screening in proliferative verrucous leukoplakia lesions.
- Source :
-
Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery [Clin Otolaryngol] 2014 Oct; Vol. 39 (5), pp. 272-80. - Publication Year :
- 2014
-
Abstract
- Objective: This study aimed to survey the presence of known oncoviruses in oral biopsies from patients diagnosed with the aetiologically undetermined proliferative verrucous leukoplakia and compare results to those from milder oral leukoplakia (OL) cases, oral squamous cell carcinoma, a common outcome of the lesions of interest, and healthy controls.<br />Design: Blind, retrospective, case-control study.<br />Setting: A stomatology unit in an academic Hospital and a Public Health laboratory.<br />Participants: Forty patients were divided in four groups. Ten patients had been diagnosed with proliferative verrucous leukoplakia, 10 with OL and 10 with OSCC, and 10 were healthy subjects.<br />Main Outcome Measures: The presence or absence of oncovirus DNA was assayed with the amplification of viral genetic markers using PCR and subsequent gel electrophoresis confirmation. Amplified fragments were sequenced and identified bioinformatically.<br />Results: No DNA from the herpesvirus, papillomavirus or polyomavirus species was detected in the samples.<br />Conclusions: No association between proliferative verrucous leukoplakia and target viruses was detected. A higher throughput viral metagenomic approach may prove valuable for future analyses, as it would not be restricted to a priori knowledge of potential targets.<br /> (© 2014 John Wiley & Sons Ltd.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Biopsy
Carcinoma, Squamous Cell pathology
Carcinoma, Squamous Cell virology
Case-Control Studies
DNA, Viral analysis
Female
Humans
Italy
Male
Middle Aged
Mouth Neoplasms pathology
Mouth Neoplasms virology
Retrospective Studies
Carcinoma, Verrucous pathology
Carcinoma, Verrucous virology
Leukoplakia, Oral pathology
Leukoplakia, Oral virology
Mass Screening
Oncogenic Viruses isolation & purification
Virus Diseases pathology
Virus Diseases virology
Subjects
Details
- Language :
- English
- ISSN :
- 1749-4486
- Volume :
- 39
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery
- Publication Type :
- Academic Journal
- Accession number :
- 25099922
- Full Text :
- https://doi.org/10.1111/coa.12291