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Activation of dopamine D2 receptor is critical for the development of form-deprivation myopia in the C57BL/6 mouse.
- Source :
-
Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2014 Aug 05; Vol. 55 (9), pp. 5537-44. Date of Electronic Publication: 2014 Aug 05. - Publication Year :
- 2014
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Abstract
- Purpose: This study used dopamine D2 receptor (D2R) knockout (KO) mice to investigate the role of D2R activity in the development of form-deprivation myopia (FDM). Sulpiride, a D2R antagonist, was administered systemically into wild-type (WT) mice to validate the involvement of D2R in FDM development.<br />Methods: The D2R KO and WT C57BL/6 mice were subjected to FDM. Wild-type mice received daily intraperitoneal injections of sulpiride, 8 μg/g body weight, for a period of 4 weeks. The body weight, refraction, corneal radius of curvature, and ocular axial components were measured at week 4 of the experiment. Differences in all ocular parameters between the experimental and control groups were compared statistically.<br />Results: Form-deprivation myopia in D2R KO mice (FD-KO) was significantly reduced compared with their WT littermates (interocular difference, -2.12 ± 0.91 diopter [D] in FD-KO versus -5.35 ± 0.83 D in FD-WT, P = 0.014), with a smaller vitreous chamber depth (0.008 ± 0.006 vs. 0.026 ± 0.006 mm, P = 0.044) and axial length (-0.001 ± 0.007 vs. 0.027 ± 0.008 mm, P = 0.007). Furthermore, FDM was attenuated in animals treated with sulpiride (-2.01 ± 0.31 D in FD-sulpiride versus -4.06 ± 0.30 D in FD-DMSO, P < 0.001) compared with those treated with vehicle, with a retardation in growth of vitreous chamber depth (-0.001 ± 0.006 vs. 0.022 ± 0.004 mm, P = 0.003) and axial length (-0.004 ± 0.007 vs. 0.027 ± 0.005 mm, P = 0.001).<br />Conclusions: Genetic and pharmacological inactivation of D2R attenuates FDM development in mice, suggesting that dopamine acting on D2R appears to promote the development of FDM in C57BL/6 mice. Further studies are required to confirm these results using animal models in which retinal D2R is selectively blocked.<br /> (Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.)
- Subjects :
- Analysis of Variance
Animals
Disease Models, Animal
Dopamine Antagonists pharmacology
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Dopamine D2 deficiency
Refraction, Ocular physiology
Sulpiride pharmacology
Form Perception physiology
Myopia physiopathology
Receptors, Dopamine D2 physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1552-5783
- Volume :
- 55
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Investigative ophthalmology & visual science
- Publication Type :
- Academic Journal
- Accession number :
- 25097246
- Full Text :
- https://doi.org/10.1167/iovs.13-13211