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Nicotine inhibits microglial proliferation and is neuroprotective in global ischemia rats.
- Source :
-
Molecular neurobiology [Mol Neurobiol] 2015; Vol. 51 (3), pp. 1480-8. Date of Electronic Publication: 2014 Aug 06. - Publication Year :
- 2015
-
Abstract
- Ischemic injury in rodent models reliably leads to the activation of microglia, which might play a detrimental role in neuronal survival. Our preliminary studies suggest that nicotine plays a potential role in decreasing the numbers of cultured microglia in vitro. In the present study, we found treatment with nicotine 2, 6, and 12 h after ischemia for 7 days significantly increased the survival of CA1 pyramidal neurons in ischemia/reperfusion rats. This effect was accompanied by a significant reduction in the increase of microglia rather than astrocytes, as well as a significant reduction of enhanced expression of tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β) in CA1 induced by ischemia/reperfusion. Nicotine inhibits microglial proliferation in primary cultures with and without the stimulation of granulocyte-macrophage colony-stimulating factor (GM-CSF). Pre-treatment with α-bungarotoxin, a selective α7 nicotinic acetylcholine receptor (α7 nAChR) antagonist, could prevent the inhibitory effects of nicotine on cultured microglial proliferation suggesting that nicotine inhibits the microglial proliferation in an α7 nAChR-dependent fashion. Our results suggest that nicotine inhibits the inflammation mediated by microglia via α7 nAChR and is neuroprotective against ischemic stroke, even when administered 12 h after the insult. α7 nAChR agonists may have uses as anti-ischemic compounds in humans.
- Subjects :
- Animals
Astrocytes drug effects
Astrocytes metabolism
Cell Survival drug effects
Interleukin-1beta metabolism
Male
Microglia metabolism
Neuroprotective Agents pharmacology
Rats, Wistar
Cell Proliferation drug effects
Ischemia metabolism
Microglia drug effects
Nicotine pharmacology
Nicotinic Agonists pharmacology
alpha7 Nicotinic Acetylcholine Receptor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1559-1182
- Volume :
- 51
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 25095782
- Full Text :
- https://doi.org/10.1007/s12035-014-8825-3