Back to Search
Start Over
Role of ornithine decarboxylase and polyamines in camostate (Foy-305)-induced pancreatic growth in rats.
- Source :
-
Digestion [Digestion] 1989; Vol. 43 (1-2), pp. 98-112. - Publication Year :
- 1989
-
Abstract
- This study was designed to investigate changes of ornithine decarboxylase and polyamines during pancreatic adaptation in response to feeding of the synthetic protease inhibitor camostate. alpha-Difluoromethylornithine, an irreversible and specific inhibitor of ornithine decarboxylase, was applied simultaneously to elucidate the essential role of polyamines in pancreatic growth. Cholecystokinin (CCK) plasma levels in camostate-fed rats increased from basal values of 3-4 pmol/l to a maximal level of 27.4 pmol/l after 2h; they then decreased up to 12 h but remained elevated above controls throughout the 30-day experiments. In the camostate group pancreatic ornithine decarboxylase activity was elevated after 2 h, reaching a maximum after 6 h (1,858.5 pmol 14CO2/h/mg DNA, about 200-fold above controls) followed by a significant increase in putrescine after 4 h and spermidine after 24 h while spermine remained unchanged. The trophic parameters increased in the following time sequence: thymidine kinase (12 h), DNA polymerase (12 h), protein (24 h), pancreatic weight (24 h) and DNA (5 days). alpha-Difluoromethylornithine significantly delayed and reduced the camostate-induced increase in ornithine decarboxylase activity and polyamine concentrations as well as the trophic parameters. Application of the CCK receptor antagonist L-364,718 resulted in complete inhibition of the increases in ornithine decarboxylase, polyamines and all trophic parameters. These data indicate an important role for ornithine decarboxylase and polyamines in camostate-induced pancreatic growth and hormonal mediated pancreatic adaptation in rats.
- Subjects :
- Animals
Cholecystokinin blood
Eflornithine pharmacology
Esters
Kinetics
Male
Ornithine Decarboxylase analysis
Pancreas analysis
Pancreas enzymology
Pancreas growth & development
Putrescine analysis
Putrescine physiology
Rats
Rats, Inbred Strains
Spermidine analysis
Spermidine physiology
Spermine analysis
Spermine physiology
Gabexate analogs & derivatives
Guanidines pharmacology
Ornithine Decarboxylase metabolism
Pancreas drug effects
Polyamines analysis
Subjects
Details
- Language :
- English
- ISSN :
- 0012-2823
- Volume :
- 43
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Digestion
- Publication Type :
- Academic Journal
- Accession number :
- 2509268
- Full Text :
- https://doi.org/10.1159/000199867