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Recombinant IκBα-loaded curcumin nanoparticles for improved cancer therapeutics.
- Source :
-
Nanotechnology [Nanotechnology] 2014 Aug 29; Vol. 25 (34), pp. 345102. Date of Electronic Publication: 2014 Aug 05. - Publication Year :
- 2014
-
Abstract
- The field of recombinant protein therapeutics has been evolving rapidly, making significant impact on clinical applications for several diseases, including cancer. However, the functional aspects of proteins rely exclusively on their structural integrity, in which nanoparticle mediated delivery offers unique advantages over free proteins. In the present work, a novel strategy has been developed where the nanoparticles (NPs) used for the delivery of the recombinant protein could contribute to enhancing the therapeutic efficacy of the recombinant protein. The transcription factor, NFκB, involved in cell growth and its inhibitor, IκBα, regulates its proliferation. Another similar naturally available molecule, which inhibits the function of NFκB, is curcumin. Hence, we have developed a 'green synthesis' method for preparing water-soluble curcumin nanoparticles to stabilize recombinant IκBα protein. The NPs were characterized by UV-vis and fluorescence spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering before administration into human cervical carcinoma (HeLa) and glioblastoma (U87MG) cells. Experimental results demonstrated that this combined module had enhanced therapeutic efficacy, causing apoptotic cell death, which was confirmed by cytotoxicity assay and flowcytometry analyses. The expression of apoptotic genes studied by semi-quantitative reverse transcription PCR delineated the molecular pathways involved in cell death. Thus, our study revealed that the functional delivery of recombinant IκBα-loaded curcumin NPs has promise as a natural-product-based protein therapeutics against cancer cells.
- Subjects :
- Antineoplastic Combined Chemotherapy Protocols therapeutic use
Apoptosis
Curcumin therapeutic use
Drug Delivery Systems
HeLa Cells
Humans
I-kappa B Proteins genetics
I-kappa B Proteins therapeutic use
NF-KappaB Inhibitor alpha
Nanoparticles chemistry
Recombinant Proteins administration & dosage
Recombinant Proteins therapeutic use
Antineoplastic Combined Chemotherapy Protocols administration & dosage
Curcumin administration & dosage
I-kappa B Proteins administration & dosage
Nanoparticles therapeutic use
Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1361-6528
- Volume :
- 25
- Issue :
- 34
- Database :
- MEDLINE
- Journal :
- Nanotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 25091588
- Full Text :
- https://doi.org/10.1088/0957-4484/25/34/345102