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ER reorganization is remarkably induced in COS-7 cells accumulating transmembrane protein receptors not competent for export from the endoplasmic reticulum.
- Source :
-
The Journal of membrane biology [J Membr Biol] 2014 Nov; Vol. 247 (11), pp. 1149-59. Date of Electronic Publication: 2014 Aug 03. - Publication Year :
- 2014
-
Abstract
- The newly synthesized mutant L501fsX533 Frizzled-4 form and the alpha3beta4 nicotinic acetylcholine receptor expressed in the absence of nicotine accumulate in the endoplasmic reticulum of COS-7 cells and induce the formation of large areas of smooth and highly convoluted cisternae. This results in a generalized block of the transport to the Golgi complex of newly synthesized proteins. Intriguingly, both effects happen peculiarly in COS-7 cells; HeLa, Huh-7, and HEK293 cells expressing the two receptors at similar level than COS-7 cells show normal ER and normal transport toward the plasma membrane. These results question the conclusion that a dominant-negative mechanism would explain the dominance of the mutant L501fsX533 Fz4 allele in the transmission of a form of Familial exudative vitreoretinopathy. Moreover, they indicate that the coordination of endoplasmic reticulum homeostasis in COS-7 cells is particularly error prone. This finding suggests that COS-7 cells may be extremely useful to study the molecular mechanisms regulating endoplasmic reticulum size and architecture.
Details
- Language :
- English
- ISSN :
- 1432-1424
- Volume :
- 247
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- The Journal of membrane biology
- Publication Type :
- Academic Journal
- Accession number :
- 25086772
- Full Text :
- https://doi.org/10.1007/s00232-014-9710-8