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Ultrashort pulsed laser treatment inactivates viruses by inhibiting viral replication and transcription in the host nucleus.
- Source :
-
Antiviral research [Antiviral Res] 2014 Oct; Vol. 110, pp. 70-6. Date of Electronic Publication: 2014 Jul 30. - Publication Year :
- 2014
-
Abstract
- Ultrashort pulsed laser irradiation is a new method for virus reduction in pharmaceuticals and blood products. Current evidence suggests that ultrashort pulsed laser irradiation inactivates viruses through an impulsive stimulated Raman scattering process, resulting in aggregation of viral capsid proteins. However, the specific functional defect(s) in viruses inactivated in this manner have not been demonstrated. This information is critical for the optimization and the extension of this treatment platform to other applications. Toward this goal, we investigated whether viral internalization, replication, or gene expression in cells were altered by ultrashort pulsed laser irradiation. Murine Cytomegalovirus (MCMV), an enveloped DNA virus, was used as a model virus. Using electron and fluorescence microscopy, we found that laser-treated MCMV virions successfully internalized in cells, as evidenced by the detection of intracellular virions, which was confirmed by the detection of intracellular viral DNA via PCR. Although the viral DNA itself remained polymerase-amplifiable after laser treatment, no viral replication or gene expression was observed in cells infected with laser-treated virus. These results, along with evidence from previous studies, support a model whereby the laser treatment stabilizes the capsid, which inhibits capsid uncoating within cells. By targeting the mechanical properties of viral capsids, ultrashort pulsed laser treatment represents a unique potential strategy to overcome viral mutational escape, with implications for combatting emerging or drug-resistant pathogens.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- 3T3 Cells
Animals
Capsid metabolism
Capsid Proteins metabolism
Capsid Proteins radiation effects
Cell Line
DNA, Viral genetics
Gene Expression radiation effects
Mice
Mice, Inbred BALB C
Transcription, Genetic radiation effects
Virus Internalization radiation effects
Low-Level Light Therapy
Muromegalovirus radiation effects
Protein Aggregates radiation effects
Virus Inactivation radiation effects
Virus Replication radiation effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-9096
- Volume :
- 110
- Database :
- MEDLINE
- Journal :
- Antiviral research
- Publication Type :
- Academic Journal
- Accession number :
- 25086212
- Full Text :
- https://doi.org/10.1016/j.antiviral.2014.07.012