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Silencing of phosphoinositide-specific phospholipase C ε remodulates the expression of the phosphoinositide signal transduction pathway in human osteosarcoma cell lines.
- Source :
-
Anticancer research [Anticancer Res] 2014 Aug; Vol. 34 (8), pp. 4069-75. - Publication Year :
- 2014
-
Abstract
- Background: Ezrin, a member of the ezrin-radixin-moesin family, is involved in the metastatic spread of osteosarcoma. Ezrin binds phosphatydil inositol-4,5-bisphosphate (PIP2), a crucial molecule of the phosphoinositide signal transduction pathway. PIP2 levels are regulated by phosphoinositide-specific phospholipase C (PI-PLC) enzymes. PI-PLCε isoform, a well-characterized direct effector of rat sarcoma (RAS), is at a unique convergence point for the broad range of signaling pathways that promote RAS GTPase-mediated signalling.<br />Materials and Methods: By using molecular biology methods and microscopic analyses, we analyzed the expression of ezrin and PLC genes after silencing of PLCE (OMIM *608414) in 143B and Hs888 cell lines.<br />Results: The growth rate of the cells was slowed, and the expression of ezrin, PLCB1, PLCG2 and PLCD4 was significantly modified. Ezrin displacement from the plasma membrane was observed.<br />Conclusion: The present results corroborate the hypothesis that ezrin and the PI signal transduction system are involved in a common network.<br /> (Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.)
- Subjects :
- Bone Neoplasms metabolism
Cell Line, Tumor
Cell Proliferation
Cytoskeletal Proteins analysis
Cytoskeletal Proteins genetics
Gene Expression Regulation, Neoplastic
Humans
Osteosarcoma metabolism
Phosphoinositide Phospholipase C analysis
Phosphoinositide Phospholipase C genetics
Phospholipase C beta genetics
Real-Time Polymerase Chain Reaction
Bone Neoplasms pathology
Osteosarcoma pathology
Phosphatidylinositols physiology
Phosphoinositide Phospholipase C physiology
Signal Transduction physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 34
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 25075031