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Topographic variations in biomechanical and biochemical properties in the ankle joint: an in vitro bovine study evaluating native and engineered cartilage.
- Source :
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Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association [Arthroscopy] 2014 Oct; Vol. 30 (10), pp. 1317-26. Date of Electronic Publication: 2014 Jul 23. - Publication Year :
- 2014
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Abstract
- Purpose: The purposes of this study were to identify differences in the biomechanical and biochemical properties among the articulating surfaces of the ankle joint and to evaluate the functional and biological properties of engineered neocartilage generated using chondrocytes from different locations in the ankle joint.<br />Methods: The properties of the different topographies within the ankle joint (tibial plafond, talar dome, and distal fibula) were evaluated in 28 specimens using 7 bovine ankles; the femoral condyle was used as a control. Chondrocytes from the same locations were used to form 28 neocartilage constructs by tissue engineering using an additional 7 bovine ankles. The functional properties of neocartilage were compared with native tissue values.<br />Results: Articular cartilage from the tibial plafond, distal fibula, talar dome, and femoral condyle exhibited Young modulus values of 4.8 ± 0.5 MPa, 3.9 ± 0.1 MPa, 1.7 ± 0.2 MPa, and 4.0 ± 0.5 MPa, respectively. The compressive properties of the corresponding tissues were 370 ± 22 kPa, 242 ± 18 kPa, 255 ± 26 kPa, and 274 ± 18 kPa, respectively. The tibial plafond exhibited 3-fold higher tensile properties and 2-fold higher compressive and shear moduli compared with its articulating talar dome; the same disparity was observed in neocartilage. Similar trends were detected in biochemical data for both native and engineered tissues.<br />Conclusions: The cartilage properties of the various topographic locations within the ankle are significantly different. In particular, the opposing articulating surfaces of the ankle have significantly different biomechanical and biochemical properties. The disparity between tibial plafond and talar dome cartilage and chondrocytes warrants further evaluation in clinical studies to evaluate their exact role in the pathogenesis of ankle lesions.<br />Clinical Relevance: Therapeutic modalities for cartilage lesions need to consider the exact topographic source of the cells or cartilage grafts used. Furthermore, the capacity of generating neocartilage implants from location-specific chondrocytes of the ankle joint may be used in the future as a tool for the treatment of chondral lesions.<br /> (Copyright © 2014 Arthroscopy Association of North America. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acids analysis
Animals
Ankle Joint chemistry
Biomechanical Phenomena
Cartilage, Articular chemistry
Cattle
Chondrocytes transplantation
Chromatography, High Pressure Liquid
Collagen analysis
Elastic Modulus physiology
Femur chemistry
Femur physiology
Fibula chemistry
Fibula physiology
Glycosaminoglycans analysis
In Vitro Techniques
Talus chemistry
Talus physiology
Tibia chemistry
Tibia physiology
Ankle Joint physiology
Cartilage, Articular physiology
Tissue Engineering
Subjects
Details
- Language :
- English
- ISSN :
- 1526-3231
- Volume :
- 30
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association
- Publication Type :
- Academic Journal
- Accession number :
- 25064757
- Full Text :
- https://doi.org/10.1016/j.arthro.2014.05.025