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Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
- Source :
-
Development (Cambridge, England) [Development] 2014 Aug; Vol. 141 (16), pp. 3255-65. Date of Electronic Publication: 2014 Jul 25. - Publication Year :
- 2014
-
Abstract
- We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.<br /> (© 2014. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Animals, Genetically Modified
Base Sequence
Birds
Cell Lineage
Chickens
Dendritic Cells cytology
Genes, Reporter
Genetic Techniques
Immune System
Introns
Molecular Sequence Data
Phagocytosis
Receptor, Macrophage Colony-Stimulating Factor metabolism
Sequence Homology, Nucleic Acid
Species Specificity
Transgenes
Yolk Sac physiology
Gene Expression Regulation, Developmental
Macrophages cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 141
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 25063453
- Full Text :
- https://doi.org/10.1242/dev.105593