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Leptospira-rat-human relationship in Luzon, Philippines.

Authors :: Villanueva SY
Saito M
Baterna RA
Estrada CA
Rivera AK
Dato MC
Zamora PR
Segawa T
Cavinta LL
Fukui T
Masuzawa T
Yanagihara Y
Gloriani NG
Yoshida S
Source :: Microbes and infection [Microbes Infect] 2014 Nov; Vol. 16 (11), pp. 902-10. Date of Electronic Publication: 2014 Jul 15.
Publication Year :: 2014
Abstract: Leptospirosis is a zoonotic infection that is caused by the pathogenic species of Leptospira. Rats are the most important reservoirs of these organisms. Our study aimed to characterize Leptospira isolates from humans and rats and elucidate the Leptospira-rat-human relationship in Luzon, Philippines. Forty strains were isolated from humans and rats. The isolates were confirmed to be Leptospira and pathogenic through rrl- and flaB-PCR, respectively. Around 73% of the isolates were found to be lethal to hamsters. Serotyping showed that there were mainly three predominant leptospiral serogroups in the study areas namely Pyrogenes, Bataviae, and Grippotyphosa. Gyrase B gene sequence analysis showed that all the isolates belonged to Leptospira interrogans. Most had 100% similarity with serovar Manilae (15/40), serovar Losbanos (8/40), and serogroup Grippotyphosa (8/40). Strains from each group had highly identical pulsed-field gel electrophoresis patterns and were further grouped as A (Pyrogenes, 14), B (Bataviae, 8), and C (Grippotyphosa, 10). Results further revealed that similar serotypes were isolated from both humans and rats in the same areas. It is suggested that these three predominant groups with highly similar intra-group PFGE patterns may have been primarily transmitted by rats and persistently caused leptospirosis in humans particularly in the Luzon islands.<br /> (Copyright © 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)