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Integrin α2β1 targeted GdVO4:Eu ultrathin nanosheet for multimodal PET/MR imaging.
- Source :
-
Biomaterials [Biomaterials] 2014 Oct; Vol. 35 (30), pp. 8649-58. Date of Electronic Publication: 2014 Jul 16. - Publication Year :
- 2014
-
Abstract
- Multifunctional nanoprobes open exciting possibilities for accurate diagnosis and therapy. In this research, we developed a (64)Cu-labeled GdVO4:4%Eu two-dimension (2D) tetragonal ultrathin nanosheets (NSs) that simultaneously possess radioactivity, fluorescence, and paramagnetic properties for multimodal imaging. The carboxyl-functionalized Eu(3+)-doped GdVO4 NSs were synthesized by a facile solvothermal reaction, followed by ligand exchange with polyacrylic acid (PAA). With ultrathin thickness of ∼5 nm and width of ∼150 nm, the carboxyl-functionalized NSs were further modified by DOTA chelator for (64)Cu labeling and Asp-Gly-Glu-Ala (DGEA) peptide for integrin α2β1 targeting. After initial evaluation of the cytotoxicity and targeting capability with PC-3 cells, the obtained multifunctional nanoprobes ((64)Cu-DOTA-GdVO4:4%Eu-DGEA) were further explored for targeted positron emission tomography (PET) and T1-weighted magnetic resonance imaging (MRI) of PC-3 tumor (prostate cancer, high integrin α2β1 expression) in vivo. Based on the strong fluorescence of the NSs, the particle distribution in mouse tissues was also determined by fluorescent microscopy. In summary, GdVO4:4%Eu NS is a potential multimodal multiscale nanoprobe that could not only be used for in vivo imaging, but also be tracked in cellular scale and ex vivo due to its fluorescent property.<br /> (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Death
Cell Line, Tumor
Cell Survival
Heterocyclic Compounds, 1-Ring chemical synthesis
Heterocyclic Compounds, 1-Ring chemistry
Humans
Male
Mice, Nude
Oleic Acid chemical synthesis
Oleic Acid chemistry
Oligopeptides chemical synthesis
Oligopeptides chemistry
Spectrometry, Fluorescence
Spectroscopy, Fourier Transform Infrared
Tissue Distribution
X-Ray Diffraction
Xenograft Model Antitumor Assays
Europium
Gadolinium
Integrin alpha2beta1 chemistry
Integrin alpha2beta1 metabolism
Magnetic Resonance Imaging
Multimodal Imaging
Nanoparticles ultrastructure
Positron-Emission Tomography
Vanadates
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 35
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 25043573
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2014.06.059