Back to Search
Start Over
Cimetidine effects on the immunosuppression induced by burn injury.
- Source :
-
International immunopharmacology [Int Immunopharmacol] 2014 Sep; Vol. 22 (1), pp. 273-6. Date of Electronic Publication: 2014 Jul 16. - Publication Year :
- 2014
-
Abstract
- Although many studies on the immune response following burn injuries have been reported, more attention has been given to the immunosuppression mechanism and mediators that shape the process of immune suppression. Specifically, information is not available concerning the immunomodulatory effects of the drugs which are involved in the immune response restoration. In this study, we investigated the effects of Cimetidine on the modulation of immune response in patients with burn injury of 20-60%. Two groups of patients were involved in this study; the patients in one group were treated with 15 mg/kg per day of Cimetidine while the patients in the other group were treated with placebo. Peripheral blood mononuclear cell (PBMC) expressing CD3, CD4, CD8, CD19 and CD3/HLA-DR was analyzed by flow cytometry. Cell proliferation assay using H3 thymidine was performed on PBMC samples. The proliferation assay showed a significant suppression of cell proliferation rate in post-burn patients (p = 0.001). We observed a significant reduction in the lymphocyte count (p = 0.001) and frequency of CD3 (p = 0.007) and CD4 (p = 0.001) T cells in post-burn patients. Also, the frequency of CD 19+ and HLA DR+ cells was increased compare to normal donors following burn injury. Treatment with Cimetidine increased the frequency of CD8+ T cells in the patient's peripheral blood. The PBMC proliferation rate was restored following the treatment with Cimetidine (p = 0.02). Our data indicates that Cimetidine may have beneficial effects on cell mediated immunity following burn injury.<br /> (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Subjects :
- Antigens, CD metabolism
Burns immunology
CD8-Positive T-Lymphocytes immunology
Cell Proliferation drug effects
Cells, Cultured
Cimetidine adverse effects
HLA-DR Antigens metabolism
Histamine H2 Antagonists adverse effects
Homeostasis drug effects
Homeostasis immunology
Humans
Immunity, Cellular drug effects
Immunophenotyping
Immunosuppression Therapy
Leukocytes, Mononuclear immunology
Lymphocyte Count
Burns drug therapy
CD8-Positive T-Lymphocytes drug effects
Cimetidine administration & dosage
Histamine H2 Antagonists administration & dosage
Leukocytes, Mononuclear drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1878-1705
- Volume :
- 22
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International immunopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 25038319
- Full Text :
- https://doi.org/10.1016/j.intimp.2014.07.003