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Identification and functional analysis of peroxiredoxin isoforms in Euglena gracilis.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (4), pp. 593-601. Date of Electronic Publication: 2014 May 21. - Publication Year :
- 2014
-
Abstract
- Euglena gracilis lacks catalase and contains ascorbate peroxidase (APX) which is localized exclusively in the cytosol. Other enzymes that scavenge reactive oxygen species (ROS) in Euglena have not yet been identified; therefore, ROS metabolism, especially in organelles, remains unclear in Euglena. The full-length cDNAs of four Euglena peroxiredoxins (EgPrxs) were isolated in this study. EgPrx1 and -4 were predicted to be localized in the cytosol, and EgPrx2 and -3 in plastids and mitochondria, respectively. The catalytic efficiencies of recombinant EgPrxs were similar to those of plant thiol-peroxidases, but were markedly lower than those of APX from Euglena. However, transcript levels of EgPrx1, -2, and -3 were markedly higher than those of APX. The growth rate of Euglena cells, in which the expression of EgPrx1 and -4 was suppressed by gene silencing, was markedly reduced under normal conditions, indicating physiological significance of Prx proteins.
- Subjects :
- Amino Acid Sequence
Cell Proliferation
Euglena gracilis cytology
Euglena gracilis genetics
Gene Knockdown Techniques
Hydrogen Peroxide metabolism
Isoenzymes chemistry
Isoenzymes deficiency
Isoenzymes genetics
Isoenzymes metabolism
Molecular Sequence Data
Peroxiredoxins chemistry
Peroxiredoxins deficiency
Peroxiredoxins genetics
Euglena gracilis enzymology
Peroxiredoxins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 78
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25036955
- Full Text :
- https://doi.org/10.1080/09168451.2014.890037