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Regulation of the differentiation of osteoblasts and osteoclasts by a hot-water extract of adzuki beans (Vigna angularis).
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2014; Vol. 78 (1), pp. 92-9. Date of Electronic Publication: 2014 Apr 10. - Publication Year :
- 2014
-
Abstract
- Osteoporosis is a global public health problem thought to be caused by an imbalance in bone metabolism. We examined in this study the 40% ethanol fraction of HP-20 resin in combination with a hot-water adzuki extract (EtEx.40) for its effect on osteoblast and osteoclast differentiation. EtEx.40-treated murine preosteoblast MC3T3-E1 cells exhibited significantly elevated alkaline phosphatase activity and mineralization. EtEx.40 facilitated osteoblast differentiation by up-regulating such osteoblast differentiation-related molecules as runt-related transcription factor 2, distal-less homeobox 5, and osterix via p38 mitogen-activated protein kinase. EtEx.40 also suppressed the formation of large tartrate-resistant acid phosphatase-positive multinucleated cells in RAW264.7 cells that had been stimulated with the receptor activator of the nuclear factor κB ligand/macrophage colony-stimulating factor. EtEx.40 significantly inhibited NF-κB activation, thus reducing the expression of such downstream molecules as c-Fos and NFATc1. Our findings suggest that EtEx.40 could be used to maintain bone mass.
- Subjects :
- Animals
Cell Line
Macrophage Colony-Stimulating Factor pharmacology
Mice
NF-kappa B metabolism
Osteoblasts drug effects
Osteoclasts drug effects
Plant Extracts isolation & purification
RANK Ligand pharmacology
Signal Transduction drug effects
Up-Regulation drug effects
p38 Mitogen-Activated Protein Kinases metabolism
Cell Differentiation drug effects
Fabaceae chemistry
Hot Temperature
Osteoblasts cytology
Osteoclasts cytology
Plant Extracts pharmacology
Water chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 78
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 25036490
- Full Text :
- https://doi.org/10.1080/09168451.2014.877182