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Comparison of two assays to determine anti-citrullinated peptide antibodies in rheumatoid arthritis in relation to other chronic inflammatory rheumatic diseases: assaying anti-modified citrullinated vimentin antibodies adds value to second-generation anti-citrullinated cyclic peptides testing.
- Source :
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BioMed research international [Biomed Res Int] 2014; Vol. 2014, pp. 198198. Date of Electronic Publication: 2014 Jun 15. - Publication Year :
- 2014
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Abstract
- Determination of anti-citrullinated peptide antibodies (ACPA) plays a relevant role in the diagnosis of rheumatoid arthritis (RA). To date, it is still unclear if the use of several tests for these autoantibodies in the same patient offers additional value as compared to performing only one test. Therefore, we evaluated the performance of using two assays for ACPA: second-generation anti-citrullinated cyclic peptides antibodies (anti-CCP2) and anti-mutated citrullinated vimentin (anti-MCV) antibodies for the diagnosis of RA. We compared three groups: RA (n = 142), chronic inflammatory disease (CIRD, n = 86), and clinically healthy subjects (CHS, n = 56) to evaluate sensitivity, specificity, predictive values, and likelihood ratios (LR) of these two assays for the presence of RA. A lower frequency of positivity for anti-CCP2 was found in RA (66.2%) as compared with anti-MCV (81.0%). When comparing RA versus other CIRD, sensitivity increased when both assays were performed. This strategy of testing both assays had high specificity and LR+. We conclude that adding the assay of anti-MCV antibodies to the determination of anti-CCP2 increases the sensitivity for detecting seropositive RA. Therefore, we propose the use of both assays in the initial screening of RA in longitudinal studies, including early onset of undifferentiated arthritis.
- Subjects :
- Adult
Antibodies blood
Antibodies immunology
Arthritis, Rheumatoid immunology
Arthritis, Rheumatoid pathology
Citrulline immunology
Female
Humans
Male
Middle Aged
Peptides, Cyclic blood
Peptides, Cyclic immunology
Rheumatic Fever immunology
Rheumatic Fever pathology
Antibodies isolation & purification
Arthritis, Rheumatoid diagnosis
Rheumatic Fever diagnosis
Vimentin immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2314-6141
- Volume :
- 2014
- Database :
- MEDLINE
- Journal :
- BioMed research international
- Publication Type :
- Academic Journal
- Accession number :
- 25025037
- Full Text :
- https://doi.org/10.1155/2014/198198