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G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus.
- Source :
-
Nature communications [Nat Commun] 2014 Jul 09; Vol. 5, pp. 4366. Date of Electronic Publication: 2014 Jul 09. - Publication Year :
- 2014
-
Abstract
- Several intracellular pathogens, such as Brucella abortus, display a biphasic infection process starting with a non-proliferative stage of unclear nature. Here, we study the cell cycle of B. abortus at the single-cell level, in culture and during infection of HeLa cells and macrophages. The localization of segregation and replication loci of the two bacterial chromosomes indicates that, immediately after being engulfed by host-cell endocytic vacuoles, most bacterial cells are newborn. These bacterial cells do not initiate DNA replication for the next 4 to 6 h, indicating a G1 arrest. Moreover, growth is completely stopped during that time, reflecting a global cell cycle block. Growth and DNA replication resume later, although bacteria still reside within endosomal-like compartments. We hypothesize that the predominance of G1-arrested bacteria in the infectious population, and the bacterial cell cycle arrest following internalization, may constitute a widespread strategy among intracellular pathogens to colonize new proliferation niches.
- Subjects :
- Brucella abortus physiology
Brucellosis genetics
Brucellosis physiopathology
Cells, Cultured
Chromosomes, Bacterial genetics
Chromosomes, Bacterial physiology
DNA Replication
DNA, Bacterial genetics
G1 Phase Cell Cycle Checkpoints genetics
HeLa Cells
Humans
Vacuoles microbiology
Vacuoles physiology
Brucella abortus cytology
Brucella abortus pathogenicity
Brucellosis pathology
G1 Phase Cell Cycle Checkpoints physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 5
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 25006695
- Full Text :
- https://doi.org/10.1038/ncomms5366