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A steroid receptor coactivator acts as the DNA-binding partner of the methoprene-tolerant protein in regulating juvenile hormone response genes.
- Source :
-
Molecular and cellular endocrinology [Mol Cell Endocrinol] 2014 Aug 25; Vol. 394 (1-2), pp. 47-58. Date of Electronic Publication: 2014 Jul 06. - Publication Year :
- 2014
-
Abstract
- Methoprene-tolerant (Met) protein is a juvenile hormone (JH) receptor in insects. JH-bound Met forms a complex with the βFtz-F1-interacting steroid receptor coactivator (FISC) and together they regulate JH response genes in mosquitoes. Both proteins contain basic helix-loop-helix (bHLH) and PAS motifs. Here we demonstrated that FISC is the obligatory partner of Met for binding to JH-response elements (JHREs). Met or FISC alone could not bind a previously characterized JHRE, while formation of the Met-FISC complex was necessary and sufficient to bind to the JHRE. This binding required participation of the DNA-binding domains of both Met and FISC. The optimal DNA sequence recognized by Met and FISC contained a core consensus sequence GCACGTG. While formation of the Met-FISC complex in mosquito cells was induced by JH, heterodimerization and DNA binding of bacterially expressed Met and FISC were JH-independent, implying that additional mosquito proteins were required to modulate formation of the receptor complex.<br /> (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Subjects :
- Aedes metabolism
Amino Acid Sequence
Animals
Base Sequence
Basic Helix-Loop-Helix Transcription Factors chemistry
Basic Helix-Loop-Helix Transcription Factors metabolism
Binding Sites
Cells, Cultured
DNA genetics
Escherichia coli genetics
Escherichia coli metabolism
Genes, Reporter
Juvenile Hormones chemistry
Juvenile Hormones metabolism
Luciferases genetics
Luciferases metabolism
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Multimerization
Protein Structure, Secondary
Protein Structure, Tertiary
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Response Elements
Sequence Alignment
Signal Transduction
Aedes genetics
Basic Helix-Loop-Helix Transcription Factors genetics
DNA metabolism
Gene Expression Regulation, Developmental
Juvenile Hormones genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8057
- Volume :
- 394
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 25004255
- Full Text :
- https://doi.org/10.1016/j.mce.2014.06.021